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MUSTELA VISON ACE2 FUNCTIONS AS A RECEPTOR FOR SARS-CORONAVIRUS Lindsay K. Heller, Laura Gillim-Ross, Emily R. Olivieri, and David E. Wentworth 1. INTRODUCTION In 2002–2003, there was an outbreak of severe acute respiratory syndrome (SARS) in Guangdong Province, China. This outbreak subsequently affected 29...
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THE MOLECULAR BIOLOGY OF CORONAVIRUSES Paul S. Masters Wadsworth Center, New York State Department of Health Albany, New York 12201 I. Introduction II. Taxonomy III. Virion Morphology, Structural Proteins, and Accessory Proteins A. Virus and Nucleocapsid B. Spike Protein (S) C. Membrane Protein (M)...
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The Molecular Biology of SARS Coronavirus NAMITA SATIJA AND SUNIL K. LAL Virology Group, International Centre for Genetic Engineering & Biotechnology, New Delhi, India ABSTRACT: Severeacuterespiratorysyndrome(SARS)isthefirstemerg- ing infectious disease of the 21st century that has been highly transmis- sible and fatal and was caused...
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BOOKS & MEDIA Travel Medicine: Tales Behind the Science Annelies Wilder-Smith, Marc Shaw, and Eric Schwartz, editors Elsevier Science, New York, New York, USA, 2007 ISBN: 978-0-08-045359-0 Pages: 333; Price: US $42.80 This book is a compilation of 40 essays written by many of...
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Role of phosphorylation clusters in the biology of the coronavirus infectious bronchitis virus nucleocapsid protein Kelly-Anne Spencer a, Michael Dee b, Paul Britton b, Julian A. Hiscox a,c,? a Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds, LS2...
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1 A Pancoronavirus RT-PCR Assay for Detection of All Known Coronaviruses Leen Vijgen, Elien Moës, Els Keyaerts, Sandra Li, and Marc Van Ranst Abstract The recent discoveries of novel human coronaviruses, including the coronavirus causing SARS, and the previously unrecognized human coronaviruses HCoV-NL63 and...
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10 Isolation and Propagation of Coronaviruses in Embryonated Eggs James S. Guy Abstract The embryonated egg is a complex structure comprising an embryo and its supporting membranes (chorioallantoic, amniotic, yolk). The developing embryo and its membranes provide the diversity of cell types that are...
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11 Large-Scale Preparation of UV-Inactivated SARS Coronavirus Virions for Vaccine Antigen Yasuko Tsunetsugu-Yokota Abstract In general, a whole virion serves as a simple vaccine antigen and often essential material for the analysis of immune responses against virus infection. However, to work with highly contagious...
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8 Titration of Human Coronaviruses, HCoV-229E and HCoV-OC43, by an Indirect Immunoperoxidase Assay Francine Lambert, Hélène Jacomy, Gabriel Marceau, and Pierre J. Talbot Abstract Calculation of infectious viral titers represents a basic and essential experimental approach for virologists. Classical plaque assays cannot be used...
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Current Immunology Reviews, 2009, 5, 267-276 267 1573-3955/09 $55.00+.00 © 2009 Bentham Science Publishers Ltd. The Biology of Persistent Infection: Inflammation and Demyelination Following Murine Coronavirus Infection of the Central Nervous System Martin P. Hosking1 and Thomas E. Lane*,1,2,3 1Department of Molecular Biology and...
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Chapter 9 The Nucleocapsid Protein of the SARS Coronavirus: Structure, Function and Therapeutic Potential Milan Surjit and Sunil K. Lal Abstract As in other coronaviruses, the nucleocapsid protein is one of the core components of the SARS coronavirus (CoV). It oligomerizes to form a...
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A NEW MODEL FOR CORONAVIRUS TRANSCRIPTION S. G. Sawicki and D. L. Sawicki Department of Microbiology and Immunology Medical College of Ohio Tole!io, Ohio 43699 I. ABSTRACT 26 Coronaviruses contain an unusually long (27-32,000 ribonucleotide) positive sense RNA genome that is polyadenylated at the...
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MOLECULAR ANALYSIS OF THE CORONA VIRUS-RECEPTOR FUNCTION OF AMINOPEPTIDASE N Andreas F. Kolb, Annette Hegyi, Julia Maile, Angelien Heister, Margitta Hagemann, and Stuart G. Siddell Institute of Virology and Immunology University ofWiirzburg, Versbacherstr. 7 D-97078 Wiirzburg, Germany 1. ABSTRACT 8 Aminopeptidase N (APN) is...
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Tick…Tick… Hear that clock ticking? It’s the countdown to the SAT Biology Ecology/Molecular Subject Test, which will be here before you know it. Whether you have a year to go or just one day, this is the time to start maximizing your score. The...
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RESEARCH MATTERS Research Driven by Curiosity: The Journey from Basic Molecular Biology and Virology to Studies of Human Pathogenic Coronaviruses Stanley Perlman* Departments of Microbiology and Pediatrics, University of Iowa, Iowa City, Iowa, United States of America *
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Coronaviruses Helena Jane Maier Erica Bickerton Paul Britton Editors Methods and Protocols Methods in Molecular Biology 1282 M E T H O D S I N M O L E C U L A R B I O L O G Y Series Editor...
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Adv Exp Med Biol - Advances in Microbiology, Infectious Diseases and Public Health DOI 10.1007/5584_2016_133 # Springer International Publishing Switzerland 2016 The Middle East Respiratory Syndrome Coronavirus – A Continuing Risk to Global Health Security Esam I. Azhar, Simone Lanini, Giuseppe Ippolito, and Alimuddin...
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269 Sunil Thomas (ed.), Vaccine Design: Methods and Protocols: Volume 1: Vaccines for Human Diseases, Methods in Molecular Biology, vol. 1403, DOI 10.1007/978-1-4939-3387-7_14, © Springer Science+Business Media New York 2016 Chapter 14 Development of a SARS Coronavirus Vaccine from Recombinant Spike Protein Plus Delta...
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59 Daniel R. Perez (ed.), Reverse Genetics of RNA Viruses: Methods and Protocols, Methods in Molecular Biology, vol. 1602, DOI 10.1007/978-1-4939-6964-7_5, © Springer Science+Business Media LLC 2017 Chapter 5 Efficient Reverse Genetic Systems for Rapid Genetic Manipulation of Emergent and Preemergent Infectious Coronaviruses Adam...
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83 Daniel R. Perez (ed.), Reverse Genetics of RNA Viruses: Methods and Protocols, Methods in Molecular Biology, vol. 1602, DOI 10.1007/978-1-4939-6964-7_6, © Springer Science+Business Media LLC 2017 Chapter 6 Reverse Genetics System for the Avian Coronavirus Infectious Bronchitis Virus Erica Bickerton, Sarah M. Keep,...
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